|CALUX||| home | about us | CALUX | services | food and feed | info | literature | news | jobs | contact |||action mode|
Frequently Asked Questions
|SWITCH ON THE LIGHT!|
BDS' innovative' CALUX® cells have been tailored so that they produce light in a dose responsive way when exposed to certain chemicals. For example: the DR CALUX® for dioxins and/or dioxin-like PCB's, the ER CALUX® for (anti)estrogens compounds and the AR CALUX® for (anti)androgen compounds. For an overview of our CALUX® cells click here.
The mechanism of action of chemicals such as PHAHs has been extensively studied over the past decades and BDS CALUX® assay mimics this mechanism in a biologically relevant way.
PCDDs, PCDFs, and PCBs share a common mode of action. These compounds bind to an intracellular receptor, known as the aryl hydrocarbon (Ah) receptor. Binding to the Ah receptor is followed by transportation of the PHAH-Ah receptor complex into the nucleus of the cell and subsequent binding to specific sequences in the DNA. The same mode of action applies for other chemicals.
These specific DNA sequences are called responsive elements (REs). Binding of the chemical-receptor complex to the RE triggers the expression of RE associated genes. The toxicological impact of the chemicals starts with the observed change in gene expression.