Title: Bioanalytical detection methods (BDMs) and monitoring

Auteur(s): Behnisch PA ; Hosoe K ; Shiozaki K ; Sakai S ;  ; 

Year: 2000

Journal: Organohalogen Compounds

Subject(s): monitoring ; bioassay ; toxicity ; PCDDs/PCDFs ; PCBs ; PCB ; 2,3,7,8-TCDD ; Reporter gene ; Reporter gene assay ; CALUX ; dioxin ; TEQ ; dioxins ; Ah receptor ; analysis ;  ; 

Summary: A battery of in vivo, in vitro bioassays and ligand binding assays have been developed to detect the toxicity of the chemical family of halogenated aromatic hydrocarbons (HAHs), such as several PCDDs/PCDFs, PCBs, PCNs and brominated analogues of the above mentioned chemical groups always in comparison to the toxicity of 2,3,7,8-TCDD. Bioanalytical detection methods (BDMs) includes the AHH/EROD bioassay, recently new developed sensitive and specific enzyme immunoassays (EIAs), reporter gene assays (CALUX), the GRAB assay, the DELFIA Dioxin TEQ assay, the filtration assay with radiolabelled dioxins and the Ah-immunoassay (AbIA). Most of BDMs for the determination of TEQs are based on the assumption that dioxin-related compounds all act through the Ah receptor (AhR) signal transduction pathway (BROD, CALUX, CAFLUX) and/or based on antibodies (EIAs). Several reviews and reports have been already published about BDMs for DLCsl-16. The aim of this review will be to describe principles and advantages/limitations of these BDMs to detect DLCs and will give some examples of their applications in field studies in comparison to the chemical analysis.