Summary: A cross-validation study was started in June 2000 analyzing 3 standards (PCB-126; 23478-PCDF; 23478-PBDF), 4 mixtures of PCBs/PCDD/Fs and 13 sample extracts of fly ashes (n=3), emission gas (n=l), PCB capacitor oils (n=2), mineral oil (n=l), sediments (n=3) and feed stuffs (n=3) at BioDetection Systems (BDS; Amsterdam, The Netherlands) and later at Kaneka Corporation (KC; Takasago, Japan). The aim was to proof the reliability of the DR-CALUX®-bioassay and to compare with the Micro-EROD-bioassay and chemical analyses.