Title: CALUX® reporter cell lines as sensitive bioassays for monitoring biological activity of various classes of steroid hormones reveal the repressive effect of TCDD on the estrogen receptor

Auteur(s): Sonneveld E ; Jansen HJ ; Riteco N ; Brouwer A ; Van der Burg B ;  ; 

Year: 2003

Journal: Organohalogen Compounds

Subject(s): bioassay ; CALUX ; estrogen ; monitoring ; dioxins ; dioxin ; PCBs ; PCB ; endocrine ; TCDD ;  ; 

Summary: BioDetection Systems B.V. (BDS) is a Dutch company providing biological detection systems for the determination of very low levels of high toxic materials such as dioxins and PCBs, and for the detection of endocrine disrupting materials such as estrogens and anti-estrogens. We recently established a range of highly specific and selective CALUX® bioassays for the detection of steroid hormones (estrogens; ERa-CALUX®, androgens; AR-CALUX®, progestins; PR-CALUX®, and glucocorticoids; GR-CALUX®). Here, we describe the characteristics of these assays, with special reference to interference by dioxins of steroid hormone signaling. While TCDD was shown to be a potent repressor of ER signaling in the ER-CALUX® bioassay containing a dioxin signaling pathway, it was without effect in the novel CALUX® bioassays.