Title: Application of the CALUXTM assay to the analysis of DXNS in a composite from sushi samples (The third report)
Auteur(s): Toyoda M ; Nishida K ; Tagata H ; Kawakami H ; Nagasaki T ; Nakamura M ; Yabushita H ; Murata H ; Tsutsumi T ; Sakai K ; ;
Journal: Organohalogen Compounds
Subject(s): analysis ; ;
Summary: The main source of dioxins (DXNs) such as polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and dioxin-like coplanar polychlorinated biphenyls (Co-PCBs) is food. From the results of a total diet study in Japan, the intake of fish and shellfish was the main contribution to the dietary exposure of DXNs. For the risk management of DXN intake from fish, frequent examination of DXN levels in fish is needed. In DXN2002, we reported that the CALUX™ (Chemically Activated Luciferase Expression) assay is a useful method for the screening and monitoring of DXNs in fish samples, with good correlation between high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) and CALUX™ (0 = 0.820). Japanese people like to eat sushi, a special dish composed of boiled and cooled rice with vinegar, and filets of many kinds of raw or cooked fish and shellfish, such as tuna, conger eel, salmon, squid, octopus and others. Our purpose was to determine the applicability of the CALUX™ assay to the rapid screening of DXN concentrations in one meal of sushi. The concentration data by CALUX™ assay in the composite mixture prepared from packed sushi samples were compared to those by the HRGC/HRMS method as a reference method.