Title: Validation of extraction, cleanup and DR CALUX bioanalysis. Part I: feedingstuff

Auteur(s): Besselink H ; Jonas A ; Pijnappels M ; Swinkels A ; Brouwer A ;  ; 

Year: 2004

Journal: Organohalogen Compounds

Subject(s): validation ; DR CALUX ; CALUX ; food ; limit-values ; dioxins ; dioxin ; bioassay ; dioxin-like compounds ; monitoring ; PCBs ; PCB ; extraction ; analysis ; clean-up ;  ; 

Summary: Food safety is a high priority issue for the food sector as it directly impacts the human health. Currently stringent EU limit values are in force for dioxins in foodstuffs for public health protection. Biodetection Systems BV's (BDS) DR CALUX® bioassay is a cost-effective and rapid method to measure low levels of dioxins and dioxin-like compounds in various matrices. The use of bioassays for monitoring dioxins in food allows the (pre)-selection of samples suspected of being contaminated above limit values with dioxins. To permit bioassays to be used for screening foodstuffs for the presence of dioxins and related compounds, the EU has laid down general requirements for the determination of dioxins and dioxin-like PCBs in foodstuffs and specific requirements for cell-based bioassays4. To ensure reliability the DR CALUX® bioassay, validated methods for extraction and DR CALUX® analysis are necessary. Within the framework of the development of DR CALUX® analysis methods, extraction and clean-up methods for 14 foodstuffs were evaluated, selected and validated. In this paper we present the results of this substantive multi-matrix foodstuff validation study.