Title: Analysis of polychlorinated dibenzo-p-dioxins (PCDD), dibenzofurans (PCDF) and biphenyls (PCB) in fish using DR-CALUX® and GC/MS: a comparison

Auteur(s): Besselink H ; Leonards P ; Felzel E ; Brouwer B ;  ; 

Year: 2002

Journal: Organohalogen Compounds

Subject(s): dioxin ; PCB ; polychlorinated ; limit-values ; food ; feed ; bioassay ; analysis ; TEQ ;  ; 

Summary: Implementation of new PCDD and PCDF limit values in food en feed products by the EU on July 2002, has increased the need for cheap, rapid, and high throughput screening techniques. A number of biological methods have been reviewed and compared by Behnisch el al. The DR-CALUX® technology was identified as the only biotechnology that meets EU quality criteria for bioassays. In comparison to chemical analysis using HRGCMS, the DR-CALUX®bioassay provide a direct measure of the total TEQ of dioxin-like activity present in a matrix, including synergistic or antagonistic effects of all dioxin-like congeners in a complex mixture. In the present study, fish samples from various sites were analysed for PCB, PCDD, and PCDF content using GC analysis. In addition, the total TEQ of dioxin-like activity was determined using the DR-CALUX® bioassay.