Title: Development of bio-monitoring tool in waste wood recycling using the CALUX assay

Auteur(s): Asari M ; Takatsuki H ; Ymamzaki M ; Azuma T ; Takigami H ; Sakai S ;  ; 

Year: 2003

Journal: Organohalogen Compounds

Subject(s): bio-monitoring ; CALUX ; waste wood ; PAH ; polychlorinated ; polychlorinated dibenzo-p-dioxins ; food ; monitoring ; dioxin ; feed ; Luciferase ; bioassay ; clean-up ; dioxin-like compounds ;  ; 

Summary: A large amount of waste wood has been and will continue to be released each year. Instead of waste incineration and landfill, recycling has been accelerated in waste wood disposal these years. In addition to paper and paperboard, fiberboard products, particleboard, another items made of recovered waste wood could be included in mulch and animal bedding. However, the use of waste wood as animal bedding poses the potential concern about hazardous contaminants not sufficiently removed from waste flows such as demolition wastes. Since animal bedding is used in direct contact with live stock and then thrown into pasture or farm soil possibly after composed, potential contaminants pose a risk of dermal, inhalant and oral exposure. Some contaminants potentially including wood preservatives such as Chromated Copper Arsenate (CCA), polycyclic aromatic hydrocarbons (PAHs), chlorophenols (CPs) and their impurities, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/DFs) have a high possibility to be accumulated in the body of cattle, resulting in hazardous effects even on humans through meat and dairy products. To control their contamination levels in food chain, an integrated and systematic approach coupled with limit-setting and monitoring for the presence of dioxin in food and feed which entered into force in EU (IP/0l/1698, IP/0l/1670) is being designed in Europe. In addition to food and feed, waste wood materials such as animal bedding may also have to be monitored to avoid contamination of food chain. The objectives of this study were to investigate the chemical characteristics of waste wood samples and to develop the CALUX (Chemical Activated LUciferase eXpression) bioassay as a rapid and cost-effective screening/monitoring method and a contributive tooi to risk management in the waste wood recycling process. Waste wood samples including litters were selected and firstly analyzed in terms of PCDD/DFs and Co-PCBs. Then it was investigated whether the CALUX bioassay was successfully applied to the waste wood samples in combination with clean-up procedure for the detection of dioxin-like compounds