Title: Determination of REP values by DR CALUX® and micro-erod for several PCDD/FS and Co-PCBs and comparison to WHO TEF values (1998)

Auteur(s): Hosoe K ; Behnisch PA ; Takigami H ; Sakai S ;  ; 

Year: 2002

Journal: Organohalogen Compounds

Subject(s): PCDD/F ; bioassay ; dioxin ; dioxin-like compounds ; environment ; 2,3,7,8-TCDD ; PCB ;  ; 

Summary: Cell based bioassays measuring AhR-dependent activity such as DR-CALUX® (www.biodetectionsystems.com) and Micro-EROD have been used to measure the effects of dioxin and dioxin-like compounds in the environment. The dioxin-like potency of a single compound is expressed as relative potency (REP). EC50 values, concentrations of compound eliciting 50 % of the maximum response by 2,3,7,8-TCDD, have widely used to determine REP values. On the other hand, it has been suggested that for chemicals that produce a do se-response curve not parallel and/or dose not exhibit the same maximal response as 2,3,7,8-TCDD, it may be more suitable to indicate activity of compounds as the 'a REP range'. In this study, as a part of the characterization of the responsiveness of the DR-CALUX®, REP values based on EC50 EC20, and EC5 values were determined for several PCDD/F and dioxin-like PCB congeners in comparison with Micro-EROD.