Title: CALUX and high resolution GC/MS analysis of dioxin-like compounds in chlorophenoxt pesticide formulations

Auteur(s): Huwe JK ; Clark GC ; Chu AC ; Garry V ;  ; 

Year: 2003

Journal: Organohalogen Compounds

Subject(s): CALUX ; dioxin-like compounds ; pesticide ; Testosterone ; polychlorinated ; polychlorinated dibenzo-p-dioxins ; PCDD/F ; Reporter gene ; bioassay ; dioxin ; TEQ ; dioxins ;  ; 

Summary: An epidemiology study to evaluate the health effects of pesticide exposure is being conducted by the University of Minnesota on a cohort of pesticide applicators from the Red River Valley of Minnesota. An initial report from a sub-cohort of this study has suggested a correlation between pesticide exposure, testosterone levels, and the sex ratio of the applicators' offspring. Because polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) have been known to effect the sex ratio of offspring and are found in various chlorinated pesticides, a number of pesticides commonly used in the Red River Valley of Minnesota were collected and assayed for dioxin-like activity by a reporter gene bioassay (CALUX). Of the twelve pesticides assayed, ten had measurable dioxin toxic equivalency (TEQ) and four had TEQs of over I ppb.1 In order to confirm the presence of PCDD/Fs and provide a congener profile of dioxins in these pesticides, we have now performed high-resolution GC/high-resolution MS (HRGC-MS)analysis on several of these same pesticides. This paper will compare the results from the CALUX bioassay to that of conventional HRGC-MS analyses.