Using the CALUX® assay to detemine the amount of chemicals in a given matrix is rapid and straightforward. After sample collection (1), a simple extraction method is used to extract the chemical content (2). The extract is cleaned-up and fractionated if desired (3), after which the clean extract is dissolved in DMSO. Meanwhile, BDS' CALUX® cells are cultured (4, 5) and finally grown in 96-well plates under standardised conditions. Once a confluent monolayer is obtained, the cells are exposed to the diluted cleaned extracts (6). After lysation and adding luciferin, the luciferase activity is quantitated using a luminometer (7).		The procedure
Detected luminescense from the analysed samples is compared to the detected luminescense from a standard curve. After data handling (8), the amount of present chemicals in the analysed sample is calculated and reported.