DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
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| By using the knowledge obtained from studying the Mode of action of dioxin
and dioxin-like compounds, BDS has designed a genetically modified cell-line
(rat-hepatoma H4IIE) that contains the firefly luciferase gene as a reporter
for the presence of PHAHs. The newly constructed cell-line still contains the
complete machinery which is involved in the Mode of action of dioxins and
dioxin-like chemicals. In addition, a plasmid (pGudLuc 1.1) has been incorporated
in the cells containing mouse DREs coupled to firefly luciferase genes. |
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 pGudLuc 1.1 |
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 Measuring of toxic effect |
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The thus created stable cell-line expresses firefly luciferase genes in addition to genes that are normally expressed in the parent cells upon exposure to dioxins or dioxin-like compounds. As a consequence, these cells emit light which can very easily be quantified. The amount of light-production is related to the amount of dioxins or dioxin-like compounds in the exposure mixture. |
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We named this new assay the DR CALUX® = Dioxin Responsive Chemical Activated Luciferase gene eXpression assay. |
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