DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
| SWITCH ON THE LIGHT! BDS' innovative DR CALUX® cells have been tailored so that they produce light in a dose responsive way when exposed to dioxins and dioxin-like chemicals. The switch that turns the light on is specific to compounds of groups of compounds that have the same action giving the DR CALUX® assay a selectivity and biological relevance that cannot be matched by instrumental techniques. |
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 Mode of action |
| And now more technically... The mechanism of action of PHAHs has been extensively studied over the past decades and BDS' DR CALUX® assay mimics this mechanism in a biologically relevant way. PCDDs, PCDFs, and PCBs share a common mode of action. These compounds bind to an intracellular receptor, known as the aryl hydrocarbon (Ah) receptor. Binding to the Ah receptor is followed by transportation of the PHAH-Ah receptor complex into the nucleus of the cell and subsequent binding to specific sequences in the DNA. These specific DNA sequences are called dioxin responsive elements (DREs). Binding of the PHAH-Ah receptor to the DRE triggers the expression of DRE associated genes. The toxicological impact of dioxins and related compounds starts with the observed change in gene expression. [back up] |
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