DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
| Using the DR CALUX® assay to
detemine the amount of dioxin or
dioxin-like compounds in a given matrix
is rapid and straightforward. After sample collection (1), a simple extraction method is used to extract the dioxins and dioxin-like chemical content (2). The extract is cleaned-up and fractionated if desired (3), after which the clean extract is dissolved in DMSO. Meanwhile, BDS' DR CALUX® cells are cultured (4, 5) and finally grown in 96-well plates under standardised conditions. Once a confluent monolayer is obtained, the cells are exposed to the diluted cleaned extracts (6-48 hrs) (6). After lysation and adding luciferin, the luciferase activity is quantitated using a luminometer (7). |
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 The procedure |
| Detected luminescense from the analysed samples is compared to the detected luminescense from a TCDD standard curve. After data handling (8), the amount of TCDD TEQs in the analysed sample is calculated and reported. | ||
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