DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
| DETECTING GENDER BENDERS - ENDOCRINE DISRUPTERS In the same way as BDS' innovative DR CALUX® bioassay, we have produced a cell line that is sensitive to materials and mixtures that act like estrogens. Our ER CALUX® cells have been tailored so that they produce light in a dose responsive way when exposed to estrogens and things that act like estrogens (pseudo-estrogens). Again, the switch that turns the light on is specific to compounds of groups of compounds that have the same action giving the ER CALUX® assay a selectivity and biological relevance that cannot be matched by instrumental techniques. |
|
 ER CALUX® | Mode of action |
More technically... The mode of action of estrogens are mediated by binding, with high affinity, to a specific estrogen receptor (ER), a member of the nuclear receptor superfamiliy. Estrogen freely enters the cell and binds to the ER, inducing heat-shockprotein 90 dissociation and homodimerisation. Following dimerisation, the ER-ligand dimer translocates to the cell nucleus and binds to specific DNA sequences called estrogen responsive elements (ERE) residing in up-stream regulatory regions of estrogen responsive genes. Binding of the ER-ligand complex to the ERE induces transcription and messenger RNA is translated into protein. The synthesis of new protein can alter cell function and results in effects on physiology. [back up] |
||
|
 |
|