DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
Using the ER CALUX® assay to determine the amount of estrogenic or anti-estrogenic substances in a given matrix is rapid and straightforward.
After sample collection, a simple extraction method is used. The extract is cleaned-up and fractionated if required, after which the clean extract is diluted in DMSO.
Meanwhile, BDS' ER CALUX® cells are cultured and finally grown in 96-well plates under standardised conditions. Once a confluent monolayer is obtained, the cells are exposed to the diluted cleaned extracts (6-24 hrs). After lysation and addition of luciferin, the luciferase activity is measured using a luminometer. Detected luminescense from the analysed samples is compared to the detected luminescense from an estradiol standard curve. After data handling, the amount of estradiol equivalents (EEQs) in the analysed sample is calculated.
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