DR CALUX®
dioxins
mode/action
bioassay
procedure
properties
ER CALUX®
estrogens
mode/action
bioassay
procedure
properties
BDS' ER-CALUX® assay is the perfect screening tool for identification and determination of the (anti)estrogenicity of pure compounds and identification of (anti)estrogenic substances in complex environmental mixtures. The advantages of the newly developed ER CALUX® bioassay as compared to traditional in vivo and in vitro assays are:
> Extremely sensitive
BDS' ER CALUX® assay is capable of detecting femtograms (10-15 grammes or quadrillionths of a gramme) of estrogenic substances.
> Rapid
Using 96-well test systems allows the rapid analysis of large numbers of samples simultaneously.
> Easy sample extraction
Techniques used to extract estrogenic substances from the matrix are relatively easy to perform.
> Small sample size
The extreme sensitivity of the ER CALUX® assay allows small sample sizes: only ml's of sample are required.
> Applicable to a wide variety of matrices
Because BDS’ ER CALUX® assay is straightforward to perform, uses small sample, gives rapid results and doesn’t need expensive apparatus, extensive (anti)estrogen analysis is cost effective.
> Applicable to a wide variety of matrices
BDS' ER CALUX® assay has successfully been used to determine estrogenicity in the following matrices:
» Surface water» Groundwater» Drinking water» Wastewater» Process aqueous effluents» Sewage and sewage sludge» Pure chemicals (pharmaceuticals, Agrochemicals, fine chemicals etc)» Urine, bile> Biologically relevant
Being cell based, BDS' ER CALUX® assay is more biologically relevant than instrumental techniques. The ER CALUX® assay operates through measuring the biological response following binding at an estrogen sensitive receptor in a living cell, the same receptor that is preserved in man, mammals, fish, birds etc. In the ER CALUX® cells, respiration and metabolisation are taking place as are the mechanisms of receptor binding. The net result is that ER CALUX® cells mimic the natural biological processes to a significant extent. This characteristic of the ER CALUX® cells makes the bioassay extremely useful in e.g. pharmaceutical research. Materials which may be agonists or antagonists at the receptor can be investigated in high rate screening.
> Analysis of total (anti)estrogenic potency in a given matrix
BDS' ER CALUX® assay is the perfect screening tool for assessing complex mixtures of (anti)estrogenic substances.
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