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Scientific publications using the CALUX® bioassay

Last update: February 26, 2014

Australia 

  • Behnisch PA., Besselink H, & Brouwer A. (2009). Usage of DR CALUX® method in crisis situation and national monitoring programs: Experiences in different countries with different food and feed matrices. DIOXIN Conference 2009. 

Belgium 

  • Koppen G, Covachi A, Van Cleuvenbergen R, Schepens PJC, Winneke G, Nelen V, & Schoeters G (2001). Comparison of CALUX®-TEQ values with PCB and PCDD/F measurements in human serum of the Flanders Environmental and Health Study (FLEHS). Toxicology Letters, 123: 59 – 67.
  • Pauwels A, Cenijn PH, Schepens PJC, & Brouwer A. (2000). Comparison of chemical-activated luciferase gene expression bioassay and gas chromatography for PCB determination in human serum and follicular fluid. Environmental Health Perspectives, 108: 553 – 557.
  • Scippo M-L, Eppe G, De Pauw E, & Maghuin-Rogister G. (2004). DR CALUX® screening of food samples: Evaluation of the quantitative approach to measure dioxin, furans and dioxin-like PCBs. Talanta, 63: 1193 – 1202. 

Canada 

  • Ayotte P, Dewailly E, Lambert GH, Perkins SL, Poon R, Feeley M, Larochelle C, & Pereg D (2005). Biomarker measurements in a coastal fish-eating population environmentally exposed to organochlorines. Environmental Health Perspectives, 113: 1318 – 1324. 

Chile 

  • Behnisch PA, Gras N, Guerrero P, Besselink H, & Brouwer A. (2010). First surveillance monitoring results of feed/food items from Chile by DR CALUX® method. DIOXIN Conference 2010.  
  • Behnisch PA., Besselink H, & Brouwer A. (2009). Usage of DR CALUX® method in crisis situation and national monitoring programs: Experiences in different countries with different food and feed matrices. DIOXIN Conference 2009.
  • Instituto de Salud Publica, Santiago de Chile (2009 – 2012). Informe de resultados de vigilancia de laboratorio. Estudio de determinacon de linea de base sobre dioxinas y PCBs presentes en alimentos. 

China

  • Yu X, Zennegg M, Engwall M, Rotander A, Larsson M, Wong MH, & Weber R. E-Waste recycling heavily contaminates a Chinese city with chlorinated, brominated and mixed halogenated dioxins. Organohalogen Compounds, 70: 813 – 816.

Czech Republic 

  • Machala M, Vondracek J, Ulrich R, Rubes J, & Sram R. (1999). Evidence for estrogenic and TCDD-like activities in extracts of blood and semen of men. Organohalogen Compounds, 42: 83. 

Denmark 

  • Laier P (2001). Using the CALUX® bioassay for screening and determination of dioxin-like compounds in human milk. Organohalogen Compounds, 54: 28 – 31.
  • Halldorsson TI, Thorsdottir I, Meltzer HM, Strøm M, & Olsen SF. (2009). Dioxin-like activity in plasma among Danish pregnant women: Dietary predictors, birth weight and infant development. Environmental Research, 109: 22 – 28. 

European Union 

  • Gizzi G, Hoogenboom LAP, von Holst C, Rose M, & Anklam E. (2005). Determination of dioxins (PCDDs/PCDFs) and PCBs in food and feed using the DR CALUX® bioassay: Results of an international validation study. Food Additives & Contaminants, 22: 472 – 481
  • Van Locoa J, Van Leeuwen SPJ, Roosa P, Carbonnelle S, de Boer J, Goeyens L, & Beernaert H. (2004). The international validation of bio- and chemical-analytical screening methods for dioxins and dioxin-like PCBs: The DIFFERENCE project rounds 1 and 2. Talanta, 63: 1169 – 1182.
  • Behnisch PA, Umlauf G, Stachel B, Felzel E, & Brouwer A. (2010). Bio/chemical analysis of sediments from the Elbe River, the North Sea and from several tributaries. DIOXIN Conference 2010. 

France 

  • Gizzi G, Hoogenboom LAP, Von Holst C, Rose M, & Anklam E. (2005). Determination of dioxins (PCDDs/PCDFs) and PCBs in food and feed using the DR CALUX® bioassay: Results of an international validation study. Food Additives & Contaminants, 22: 472 – 481.

Germany 

  • Behnisch PA. (2011). Dioxins & Robotics: Innovative rapid HTPS dioxin/PCB biodetection systems. International Dairy Magazine, 9: 29 – 30.
  • Von der Trenck KT, Schilling F, Schmidt D, Behnisch PA, & Brouwer A. (2008). Organohalogen compounds in the eggs of peregrine falcons and other wild bird species in Baden-Wurttemberg - Present state and time trend. Organohalogen Compounds, 70: 145 – 148.
  • Keiter S, Grund S, Van Bavel B, Hagberg J, Engwall M, Kammann U, Klempt M, Manz W, Olsman H, Braunbeck T, et al. (2008). Activities and identification of aryl hydrocarbon receptor agonists in sediments from the Danube River. Analytical and Bioanalytical Chemistry, 390: 2009 – 2019. 

Hong Kong

  • Soechitram SD, Chan SM, Nelson EA, Brouwer A, & Sauer PJ. (2003).Comparison of dioxin and PCB concentration in human breast milk between Hong Kong and The Netherlands. Food Additives & Contaminants, 20: 65 – 69.

Italy

  • Porpora MG, Medda E, Abballe A, Bolli S, De Angelis I, Di Domenico A, Ferro A, Ingelido AM, Maggi A, Panici PB, & De Felip E. (2009). Endometriosis and organochlorinated environmental pollutants: A case-control study on Italian women of reproductive age. Environmental Health Perspectives, 117: 1070 – 1075.
  • Sciuto S, Martucci F, Desiato R, Mazza M, Guglielmetti C, Dell'Atti L, Ubaldi A, Ru G, Acutis PL, Caramelli M. (2013). Evaluation of the DR CALUX® bioassay as screening method for the detection of contamination by dioxins in milk in Piedmont. Large Animal Review, 19: 107 – 114.

Japan

  • Suzuki G, Takigami H, Kushi Y, & Sakai S. (2004). Evaluation of mixture effects in a crude extract of compost using the CALUX® bioassay and HPLC fractionation. Environment International, 30: 1055 – 1066.
  • Suzuki G, Takigami H, Nose K, Takahashi S, Asari M, & Sakai S. (2007). Dioxin-like and transthyretin-binding compounds in indoor dusts collected from Japan: Average daily dose and possible implications for children. Environmental Science & Technology, 41: 1487 – 1493.
  • Takigami H, Hoesoe K, Behnisch PA, Shiozaki K, Mizukami H, Ohno M, & Sakari S. (2002). Validation study for practical bio-monitoring of waste PCB samples during their destruction treatment using DR CALUX® assay and PCB immunoassay. Organohalogen Compounds, 58: 397 – 400.
  • Takigami H, Sakai S, & Brouwer A. (2005). Bio/chemical analysis of dioxin-like compounds in sediment samples from Osaka Bay, Japan. Environmental Technology, 26: 459 – 469. 

Kuwait 

  • Husain A, Gevao B, Dashti B, Brouwer A, Behnisch PA, Al-Wadi M, & Al-Foudari M. (2014). Screening for PCDD/Fs and dl-PCBs in local and imported food and feed products available across the State of Kuwait and assessment of dietary intake. Ecotoxicology and Environmental Safety, 100: 27 – 31.

Norway

  • Behnisch PA., Besselink H, & Brouwer A. (2009). Usage of DR CALUX® method in crisis situation and national monitoring programs: Experiences in different countries with different food and feed matrices. DIOXIN Conference 2009.

Poland

  • Behnisch PA, Umlauf G, Stachel B, Felzel E, & Brouwer A. (2010). Bio/chemical analysis of sediments from the Elbe River, the North Sea and from several tributaries. DIOXIN Conference 2010.

Slovakia

  • Behnisch PA., Besselink H, & Brouwer A. (2009). Usage of DR CALUX® method in crisis situation and national monitoring programs: Experiences in different countries with different food and feed matrices. DIOXIN Conference 2009.

Spain

  • Yunta M, Encarnación M, Pardo Marín O, Yusà Pelechà V (2013). Probabilistic assessment of the dietary exposure of the population of the Region of Valencia to dioxins. Universidad de Alicante. rua.ua.es/dspace/handle/10045/28781

Sweden

  • Engwall M, Lindstrom G, & Van Bavel B. (2003). Experiences from an international intercalibration of dioxin-like compounds in cod liver using bioassays. Organohalogen Compounds, 60: 2091 – 2094. 

Switzerland 

  • Wenger E, Gerecke AC, Heeb NV, & Zenobi R. (2006). Estrogenic and dioxin-like activity in diesel exhaust. Organohalogen Compounds, 68: 1295 – 1298. 

Taiwan

  • Shyu TS, Lee YH, Lee ML, Lu SY, Chu C, Wu CI, & Kao CW. (2009). Determination of PCDDS/DFs and dioxin-like PCBs in egg and fish/feed samples in Taiwan by the DR CALUX® bioassay. Organohalogen Compounds, 71: 434 – 438.

The Netherlands

  • Bovee TF, Hoogenboom LAP, Hamers AR, Traag WA, Zuidema T, Aarts JM, Brouwer A, & Kuiper HA. (1998). Validation and use of the CALUX® bioassay for the determination of dioxins and PCBs in bovine milk. Food Additives & Contaminants, 15: 863 – 875.
  • Hoogenboom R, Bovee T, Traag WA, Hoogerbrugge R, Baumann B, Portier L, Van de Weg G, & De Vries J. (2006). The use of the DR CALUX® bioassay and indicator polychlorinated biphenyls for screening of elevated levels of dioxins and dioxin-like polychlorinated biphenyls in eel. Molecular Nutrition & Food Research, 50: 945 – 957.
  • Van Leeuwen SPJ, Leonards PEG, Traag WA, Hoogenboom LAP, & De Boer J. (2007). Polychlorinated dibenzo-p-dioxins, dibenzofurans and biphenyls in fish from the Netherlands: Concentrations, profiles and comparison with DR CALUX® bioassay results. Analytical and Bioanalytical Chemistry, 389: 321 – 333.
  • Besselink HT, Schipper C, Klamer H, Leonards P, Verhaar H, Felzel E, Murk AJ, Thain J, Hoesoe K, Schoeters G, Legler J, & Brouwer A. (2004). Intra- and interlaboratory calibration of the DR CALUX® bioassay for the analysis of dioxins and dioxin-like chemicals in sediments. Environmental Toxicology and Chemistry, 23: 2781 – 2789.
  • Besselink HT, Felzel E, Jonas A, & Brouwer A. (2006). Results of the first International Interlaboratory DR CALUX® by BDS comparison study for food and feed (BICS 2005). Organohalogen Compounds, 68: 249 – 252.
  • Hoogenboom LAP, Kotterman MJJ, Hoek-van Nieuwenhuizen M, Van Lee ML, & Van Leeuwen SPJ (2013). Dioxins and PCBs in Chinese mitten crabs from Dutch rivers and Lakes. DIOXIN 2013 Daegu, Korea.
  • Traag WA, Van Dam G, Immerzeel J, & Hoogenboom LAP. (2013). A pecular case of PCB contamination in young organic hens. DIOXIN 2013, Daegu, Korea.
  • Reeuwijk NM, Talidda A, Malisch R, Kotz A, Tritsher A, Fiedler H, Zeilmaker MJ, Kooijman M, Wienk KJH, Traag WA, & Hoogenboom LAP. (2013). Dioxins (polychlorinated dibenzo-p-dioxins and polychlorinated dibenzo-furans) in traditional clay products used during pregnancy. Chemosphere, 90: 1678 – 1685.
  • Van Leeuwen SPJ, Kotterman MJJ, Hoek-van Nieuwenhuizen M, Van Lee MK, & Hoogenboom, LAP. (2013). Dioxines en PCB's in rode aal uit Nederlandse binnenwateren: Resultaten tussen 2006 en 2012. RIKILT Wageningen UR (Rapport / RIKILT 2013.010).
  • Hoogenboom R, Portier L, Oegema G, Immerzeel J, & Traag WA. (2012). Practical experience with the DR CALUX® assay for screening of food of animal origin. Poster DIOXIN 2011.

Ukraine

  • Brouwer A, Botschuiver S, Veerhoek D, Besselink HT, Hamm S, Traag WA, & Zimpfer M. (2005). Observation of an extremely high dioxin level in a human serum sample from Ukraine by DR CALUX® which was confirmed to be 2,3,7,8-tetrachlorodibenzo-p-dioxin by GC-HRMS. Organohalogen Compounds, 67: 1705 – 1708.

United Kingdom

  • Hurst MR, Balaam J, Chan-Man JL, Thain JE, & Thomas KV. (2004). Determination of dioxin and dioxin-like compounds in sediments from UK estuaries using a bio-analytical approach: Chemical-activated luciferase expression (CALUX®) assay. Marine Pollution Bulletin, 49: 648 – 658.
  • Air V, Pless-Mulloli T, Schilling B, Paepke O, & Foster K. (2002). Follow-up assessment of PCDD/PCDF in eggs from allotments in New Castle upon Tyne England. Organohalogen Compounds, 57: 237 – 240. 

USA 

  • Archer JC, Mobley RJ, Shojaee S, Pence LM, & Earnheart C. (2003). Alternate extraction and clean-up for CALUX® analyses of feeds and feed components. Organohalogen Compounds, 60: 207 – 210.