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Available assays

In addition to the CALUX® assays that are currently available, BDS is constantly working on new reporter cell lines and new applications with these cell lines.

Please find a detailed elaboration on all available assays in our catalogue.

We welcome you to contact Bart van der Burg for further questions and suggestions. 

Table 1. Overview of HTS CALUX® panel

Name

Pathway/Effect

Reference Compound

Key Reference

DR CALUX®

Xenobiotics metabolism / dioxin receptor activation 

2,3,7,8-TCDD

Aarts et al., 1995

PAH CALUX®

Xenobiotics metabolism / dioxin receptor activation 

benzoapyrene

Pieterse et al., 2013

ER CALUX®

Estrogen signaling

17β-estradiol

Legler et al., 1999

ERα CALUX®

Estrogen receptor α-mediated signaling

17β-estradiol

Sonneveld et al., 2005

OECD, unpublished

ERα-anti CALUX®

Repression of estrogen receptor α-mediated signaling

tamoxifen

Van der Burg et al., 2010a

OECD, unpublished

ERβ CALUX®

Estrogen receptor β-mediated signaling

17β-estradiol

Van der Burg et al., 2013

ERβ-anti CALUX®

Repression of  estrogen receptor β-mediated signaling

tamoxifen

AR CALUX®

Androgen receptor activation

dihydrotestosterone

Sonneveld et al., 2005;

OECD, unpublished

AR-anti CALUX®

Repression androgen receptor activation

flutamide

Van der Burg et al., 2010b;

OECD, unpublished

PR CALUX®

Progesterone receptor-mediated signaling

progesterone

Sonneveld et al., 2005

PR-anti CALUX®

Repression of progesterone receptor-mediated signaling

RU486

GR CALUX®

Glucocorticoid receptor-mediated signaling

dexamethasone

Sonneveld et al., 2005

anti-GR CALUX®

Repression of glucocorticoid receptor-mediated signaling

RU486

TR CALUX®

Thyroid receptor-mediated signaling

T3

Piersma et al., 2013;

Van der Burg et al., 2013

RAR CALUX®

Retinoic acid receptor-mediated signaling

retinoic acid

Piersma et al., 2013;

Van der Burg et al., 2013

PPARγ CALUX®

PPARγ-mediated signaling

rosiglitazone

Gijsbers et al., 2011

PPARα CALUX®

PPARα-mediated signaling

GW7674

Gijsbers, submitted

PPARδ CALUX®

PPARδ-mediated signaling

L165041

Szalowska et al., 2014

LXR CALUX®

LXR-mediated signaling

GW3965

unpublished

kappaB CALUX®

NFκB-mediated signaling

TPA

Piersma et al., 2013;

Van der Burg et al., 2013

P21 CALUX®

Transcription of p21 inhibitor of cell cycle progression

actinomycin D

Piersma et al., 2013;

Van der Burg et al., 2013

Nrf2 CALUX®

Activation of the Nrf2 pathway / oxidative stress response

curcumin

Van der Linden et al., 2014

P53 CALUX®

p53-dependent pathway activation / genotoxicity response (+/- S9)

actinomycin D

Van der Linden et al., 2014

genotox CALUX®

p53-dependent pathway activation +/-S9

cyclophosphamide

Van der Linden et al., 2014

TCF CALUX®

wnt/TCF pathway activation

lithium chloride

Piersma et al., 2013;

Van der Burg et al., 2013

AP1 CALUX®

AP1 pathway activation / cell cycle control

TPA

Piersma et al., 2013;

Van der Burg et al., 2013

Hif1α CALUX®

Chemical hypoxia response

cobaltous chloride

Piersma et al., 2013;

Van der Burg et al., 2013

ER stress CALUX®

Endoplasmic reticulum stress response

tunicamycin

Piersma et al., 2013;

Van der Burg et al., 2013

cytotox CALUX®

Repression of constitutive transcriptional activation

Tributyltin acetate

Van der Linden et al., 2014