Available assays

In addition to the CALUX® assays that are currently available, BDS is constantly working on new reporter cell lines and new applications with these cell lines.

Please find a detailed elaboration on all available assays in our catalogue.

We welcome you to contact Bart van der Burg for further questions and suggestions.

**Table 1. Overview of HTS CALUX**® **panel**
Name	Pathway/Effect	Reference Compound	Key Reference
DR CALUX®	Xenobiotics metabolism / dioxin receptor activation	2,3,7,8-TCDD	Aarts et al., 1995
PAH CALUX®	Xenobiotics metabolism / dioxin receptor activation	benzoapyrene	Pieterse et al., 2013
ER CALUX®	Estrogen signaling	17β-estradiol	Legler et al., 1999
ERα CALUX®	Estrogen receptor α-mediated signaling	17β-estradiol	Sonneveld et al., 2005; OECD, unpublished
ERα-anti CALUX®	Repression of estrogen receptor α-mediated signaling	tamoxifen	Van der Burg et al., 2010a; OECD, unpublished
ERβ CALUX®	Estrogen receptor β-mediated signaling	17β-estradiol	Van der Burg et al., 2013
ERβ-anti CALUX®	Repression of estrogen receptor β-mediated signaling	tamoxifen
AR CALUX®	Androgen receptor activation	dihydrotestosterone	Sonneveld et al., 2005; OECD, unpublished
AR-anti CALUX®	Repression androgen receptor activation	flutamide	Van der Burg et al., 2010b; OECD, unpublished
PR CALUX®	Progesterone receptor-mediated signaling	progesterone	Sonneveld et al., 2005
PR-anti CALUX®	Repression of progesterone receptor-mediated signaling	RU486
GR CALUX®	Glucocorticoid receptor-mediated signaling	dexamethasone	Sonneveld et al., 2005
anti-GR CALUX®	Repression of glucocorticoid receptor-mediated signaling	RU486
TR CALUX®	Thyroid receptor-mediated signaling	T3	Piersma et al., 2013; Van der Burg et al., 2013
RAR CALUX®	Retinoic acid receptor-mediated signaling	retinoic acid	Piersma et al., 2013; Van der Burg et al., 2013
PPARγ CALUX®	PPARγ-mediated signaling	rosiglitazone	Gijsbers et al., 2011
PPARα CALUX®	PPARα-mediated signaling	GW7674	Gijsbers, submitted
PPARδ CALUX®	PPARδ-mediated signaling	L165041	Szalowska et al., 2014
LXR CALUX®	LXR-mediated signaling	GW3965	unpublished
kappaB CALUX®	NFκB-mediated signaling	TPA	Piersma et al., 2013; Van der Burg et al., 2013
P21 CALUX®	Transcription of p21 inhibitor of cell cycle progression	actinomycin D	Piersma et al., 2013; Van der Burg et al., 2013
Nrf2 CALUX®	Activation of the Nrf2 pathway / oxidative stress response	curcumin	Van der Linden et al., 2014
P53 CALUX®	p53-dependent pathway activation / genotoxicity response (+/- S9)	actinomycin D	Van der Linden et al., 2014
genotox CALUX®	p53-dependent pathway activation +/-S9	cyclophosphamide	Van der Linden et al., 2014
TCF CALUX®	wnt/TCF pathway activation	lithium chloride	Piersma et al., 2013; Van der Burg et al., 2013
AP1 CALUX®	AP1 pathway activation / cell cycle control	TPA	Piersma et al., 2013; Van der Burg et al., 2013
Hif1α CALUX®	Chemical hypoxia response	cobaltous chloride	Piersma et al., 2013; Van der Burg et al., 2013
ER stress CALUX®	Endoplasmic reticulum stress response	tunicamycin	Piersma et al., 2013; Van der Burg et al., 2013
cytotox CALUX®	Repression of constitutive transcriptional activation	Tributyltin acetate	Van der Linden et al., 2014